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KMID : 1134820190480020206
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 2 p.206 ~ p.214
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Effect of Green Lipped Mussel Extract Oil Complex (Gwanjeolpalpal) on Monosodium Iodoacetate-Induced Osteoarthritis in Animal Model
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Cho Se-Haeng
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Abstract
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This study examined the effect of green lipped mussel extract oil complex (GLMOC; green lipped mussel oil, olive oil, and D-α-tocopherol) on the joints and cartilage in an osteoarthritis-induced animal model. A total of 40 seven-week-old Sprague-Dawley rats were divided randomly into 5 groups; 1) normal, 2) control, 3) GLMOC 32.5 mg/kg, 4) GLMOC 65 mg/kg, 5) GLMOC 130 mg/kg. Osteoarthritis was induced by injection of monosodium iodoacetate (MIA) into the knee joints of the rats. The rats were sacrificed at the end of 4th week after daily administration of GLMOC and then rat tibial articular cartilage was removed. Serum NF-κB was measured by ELISA and the expression levels of type Ⅰ, Ⅱ collagen, matrix-metalloproteinase (MMP)-2, 9 mRNA were analyzed by qPCR. The morphological changes in the joint were evaluated by micro-CT and staining with H&E and Safranin-O & Fast Green. No adverse events were observed during the experiment period. GLMOC significantly inhibited serum NF-κB level in rats with MIA-induced osteoarthritis. Expression of the catabolic genes of MMP-2 and MMP-9 were lower in the cartilage of the animals fed GLMOC. A histological examination of animals showed that GLMOC administration improved the lesions significantly. Bone volume fraction (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) were increased in rats fed GLMOC compared to the control group and bone surface/bone volume (BS/BV) was decreased significantly. These results suggest that GLMOC effectively protects the joints and cartilage by suppressing inflammatory responses and cartilage destruction.
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KEYWORD
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green lipped mussel extract oil complex, Gwanjeolpalpal, osteoarthritis, cartilage
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